Figure 1: Description and proof of principle for balanced PCR. As an alternative to the separation PCR step, LN1- and LN2-ligated plasmid DNA were mixed in different proportions and used in a ...

Genome editing using designer nucleases such as transcription activator-like effector nucleases (TALENs) or clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 nucleases is an ...

A team of UK-based scientists has recently developed an allele-specific probe polymerase chain reaction method that can accurately detect different variants/lineages of severe acute respiratory ...

PCR is a technique used to amplify target DNA in a sample. It’s a well-known method that has undergone numerous modifications to enhance its capabilities. This year, it’s turning 40 years old. PCR has ...

PCR or Polymerase Chain Reaction is a revolutionary method that is used to amplify specific segments of DNA or RNA. This method was developed by Kary Mullis in the 1980s. Kary Mullis received the ...

Today, PCR is ubiquitous in molecular biology laboratories. As one of the first experiments that young scientists master, it is easy to take for granted. But developing this research lab staple ...

Real-time PCR (qPCR) and digital PCR (dPCR) are both powerful techniques used for precise nucleic acid quantification, but each offers unique advantages depending on various factors. Understanding ...

Currently established rapid methods for sterility testing of advanced therapy medicinal products (ATMPs) are mostly based on growth-based processes or the detection of metabolic activities. However, ...

PCR genetic analysis has been in the spotlight since COVID-19, but light is now further facilitating PCR-free methods. Osaka Metropolitan University scientists have developed a light-induced DNA ...

Increased tracing, testing and isolation of people with novel coronavirus infection is an effective way to contain the virus spread. Currently, the preferred protocol for testing uses the RT-PCR ...

Kary B. Mullis, Nobel Laureate in Chemistry, died Aug. 7 of pneumonia at the age of 74. He won the 1993 Nobel Prize in Chemistry for his invention of the polymerase chain reaction (PCR) method for ...