This article presents a simple and systematic approach to HPLC method development, beginning with sample preparation and finishing with practical analytical method validation. step 5 - method ...

High-performance liquid chromatography (HPLC) is an analytical chemistry technique used to separate liquid mixtures of compounds or analytes. The method employs a liquid "mobile phase" to transport ...

Modern HPLC method development is dominated by a small number of pH adjusting reagents and buffers that are prevalent even when the method uses UV detection. This is driven primarily by the ...

We recently introduced a new series of UHPLC columns with a particle size of 1.6 µm. Here we explain how to transfer an existing HPLC method to a new UHPLC method using polyphenols as an example.

High Performance Liquid Chromatography (HPLC) is an analytical procedure for separation, identification and quantification of substances using liquid chromatography (LC). The mixture for separation is ...